Development of a recombinase-aided amplification assay for rapid detection of human norovirus GII.4

Abstract Background Human noroviruses are one of the main causes foodborne illnesses and represent a serious public health concern. Rapid sensitive assays for human norovirus detection undoubtedly necessary clinical diagnosis, especially in regions without more sophisticated equipment. Method The rapid reverse transcription recombinase-aided amplification (RT-RAA) is fast, robust isothermal nucleic acid method based on enzyme reaction. This can complete sample at 39 °C 30 min. In this study, we successfully established assay GII.4 applied to samples, as well comparison with commercial real-time fluorescence quantitative PCR (RT-qPCR). Results At 95% probability, sensitivity RT-RAA was 3.425 log10 genomic copies (LGC)/reaction. Moreover, no cross-reaction observed other genogroups common viruses. Stool samples were examined by polymerase chain reaction Compared RT-qPCR, kappa values 0.894 ( p < 0.001), indicating that both agreement. Conclusion provides rapid, specific, suitable testing.